Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP) and ChIP assay (ChIP) applications. Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. The region of AKT2 surrounding S474 has a high degree of similarity to the corresponding regions in AKT1 and AKT3 and thus may cross react with these proteins if phosphorylated on … Akt2 causes TGFß-induced deptor downregulation facilitating mTOR to drive podocyte hypertrophy and matrix protein expression. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Akt is activated in res Temperature: 4°C, Western blot - Anti-AKT2 (phospho S474) antibody (ab38513), Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT2 (phospho S474) antibody (ab38513), This image is courtesy of an anonymous Abreview. By signing up, you indicate that you have read and agree to the terms in our privacy policy. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-AKT1/AKT2/AKT3 (Ser124) polyclonal antibody … Get resources and offers direct to your inbox. Supplied as 100 µg purified antibody … Select appropriate Akt2 antibodies for your research by isotype, epitope, applications and species reactivity. This antibody reacts with Human, Mouse, Rat samples. Select Akt2 antibodies from monoclonal antibodies listed below. Western blot analysis of extracts from LNCaP cells, transfected with a construct expressing Akt1 shRNA or Akt2 shRNA, uninduced (-) or doxycycline-induced for the indicated times, using Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific) (top), Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 … Your email subscription to News & Announcements has been received. This antibody reacts with Human, Mouse, Non-human primate, Rat samples. View detailed Akt2 antibody specifications by linking to the specific product blocks. Invitrogen Anti-Phospho-AKT2 (Ser474) Polyclonal, Catalog # PA5-36819. AKT, or PKB, is a serine/threonine-protein kinase that is involved in regulating metabolism, … Immunofluorescent analysis of Phospho-AKT1/AKT2/AKT3 (Ser124) in HepG2 cells(4h of LPS treatment). Call us Toll Free at 1-800-457-3801. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Select appropriate Akt2 antibodies for your research by isotype, epitope, applications and species reactivity. Santa Cruz Biotechnology, Inc. offers a broad range of Akt2 antibodies. Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. Antibody Sampler Kit for studying Akt1/Akt1 (Ser473) phosphate/Akt1 (Thr308) phosphate/Akt2/Akt2 (Ser474) phosphate/Akt2 (Thr309) phosphate/Akt3/Akt3 (Ser472) phosphate/Akt3 (Thr305) phosphate/c-Raf (Ser259) phosphate/GSK3-beta (Ser9) ... Each phospho-specific antibody recognizes the phosphorylated form of its target. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Epithelial mesenchymal transition induced by the CXCL9/CXCR3 axis through AKT activation promotes invasion and metastasis in tongue squamous cell carcinoma. Supplied as 100 µL purified antibody (1 mg/mL). Murine thymoma viral (v-akt) homolog 2 antibody, murine thymoma viral (v-akt) homolog-2 antibody, Oncogene AKT2 protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase antibody, v akt murine thymoma viral oncogene homolog 2 antibody, V-AKT murine thymoma viral oncogene homolog 2 antibody. Invitrogen Anti-AKT2 Monoclonal (4H7), Catalog # MA1-034. To place an order using RMB or to ship to mainland China, please visit www.scbio.cn, PROTEIN A, G-PLUS & L AGAROSE IP REAGENTS, NUCLEOTIDES, PEPTIDES, PROTEINS & AMINO ACIDS, AUTORADIOGRAPHY FILM, TAPE AND PLASTIC WRAP, Akt2 CRISPR Knockout, HDR and Nickase Knockout Plasmids, Akt2 siRNA, shRNA Plasmid and shRNA Lentiviral Particles gene silencers, Akt1 Lentiviral Activation Particles (h2), Akt2 Lentiviral Activation Particles (h2), Akt2 Lentiviral Activation Particles (m2). All rights reserved. You have the right to unsubscribe at any time by clicking the 'unsubscribe' link in any News & Announcement emails you receive. To the left of the product names in the table below, indicates those antibodies for which a 10 µg trial size SAMPLE is available free of charge. Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474. Santa Cruz Biotechnology, Inc. offers a broad range of Akt2 antibodies. All lanes : Anti-AKT3 (phospho S472) + AKT2 (phospho S474) + AKT1 (phospho S473) antibody [EPR18853] (ab192623) at 1/1000 dilution Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) treated with 0.1 uM Calyculin A for 30 minutes whole cell lysates Lane 3 : A549 (Human lung carcinoma epithelial cell) … why is the actual band size different from the predicted? "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.